Coding

Part:BBa_K4488004

Designed by: Sylvie Nolf   Group: iGEM22_Sydney_Australia   (2022-09-30)


Anti-GFP Nanobody/sdAb

Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. GenBank ID: AIF28007 Includes an Esp3I recognition site at the end.


modelling of Nanobody 7 (AIF28007) to sfGFP created using the python package Lightdock

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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