Part:BBa_K4482016
HIRMAb-NT3
The insulin receptor is a transmembrane protein expressed in many different tissues in the human body, including endothelial cells forming the blood vessels in the brain. The insulin receptor takes an active part in transporting insulin from the blood stream into the brain through the Blood-Brain Barrier (BBB). This property of the insulin receptor has been harnessed to for the active transport of therapeutic proteins through the BBB. The human insulin receptor monoclonal antibody (HIRMAb) was developed to hitchhike into the insulin receptor and be transported through the BBB to deliver cargo into the brain when injected intravenously. Antibodies are immunoglobulins formed by two different peptides, a heavy chain and a light chain, that complex together in couples to form the mature antibody capable of recognizing its specific antigen. This part represents the coding sequence for the HIRMAb recombinant antibody. The sequence was derived from the protein sequence registered in US patent US-8858950-B2. Neurotrophin-3 is a protein that in humans is encoded by the NTF3 gene. The protein encoded by this gene, NT-3, is a neurotrophic factor in the NGF (Nerve Growth Factor) family of neurotrophins. It is a protein growth factor that has activity on certain neurons of the peripheral and central nervous system; it helps to support the survival and differentiation of existing neurons and encourages the growth and differentiation of new neurons and synapses. NT-3 was the third neurotrophic factor to be characterized, after nerve growth factor (NGF) and BDNF (Brain Derived Neurotrophic Factor). Although the vast majority of neurons in the mammalian brain are formed prenatally, parts of the adult brain retain the ability to grow new neurons from neural stem cells; a process known as neurogenesis. Neurotrophins are chemicals that help to stimulate and control neurogenesis. NT-3 is unique in the number of neurons it can potentially stimulate, given its ability to activate two of the receptor tyrosine kinase neurotrophin receptors (TrkC and TrkB). NT-3 is unique in the number of neurons it can potentially stimulate, given its ability to activate two of the receptor tyrosine kinase neurotrophin receptors (TrkC and TrkB. (Source: wikipedia). NT-3 was fused to the C-terminus of HIRMAb so that it could be transported from the blood stream through the BBB into the brain.
Sequence and Features
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Illegal BamHI site found at 537
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Illegal XbaI site found at 2330
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Illegal SpeI site found at 2585
Illegal PstI site found at 1913
Illegal PstI site found at 3719
Illegal PstI site found at 3885 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1024
Illegal EcoRI site found at 2476
Illegal EcoRI site found at 2569
Illegal XbaI site found at 2330
Illegal XbaI site found at 4311
Illegal SpeI site found at 821
Illegal SpeI site found at 2585
Illegal PstI site found at 1913
Illegal PstI site found at 3719
Illegal PstI site found at 3885 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 598
Illegal SapI.rc site found at 1427
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