Coding

Part:BBa_K4482015

Designed by: Mustafo Mustafokulov   Group: iGEM22_NYC_Empire_State   (2022-10-10)


HIRMAb-FGF2

Molecular Trojan horses are genetically engineered proteins that cross the blood-brain barrier (BBB) via endogenous receptor-mediated transport processes. Molecular Trojan horses provide a brain drug targeting technology that allows for the non-invasive delivery of large molecule therapeutics to the human brain. This sequence codes for the fusion protein of HIRMAb and FGF2 for the delivery of FGF2 to the brain through the BBB.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3966
    Illegal SpeI site found at 821
    Illegal PstI site found at 2075
    Illegal PstI site found at 2081
    Illegal PstI site found at 3374
    Illegal PstI site found at 3540
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 821
    Illegal PstI site found at 2075
    Illegal PstI site found at 2081
    Illegal PstI site found at 3374
    Illegal PstI site found at 3540
    Illegal NotI site found at 3955
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 537
    Illegal BamHI site found at 1901
    Illegal BamHI site found at 1972
    Illegal BamHI site found at 3277
    Illegal XhoI site found at 575
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3966
    Illegal SpeI site found at 821
    Illegal PstI site found at 2075
    Illegal PstI site found at 2081
    Illegal PstI site found at 3374
    Illegal PstI site found at 3540
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3966
    Illegal SpeI site found at 821
    Illegal PstI site found at 2075
    Illegal PstI site found at 2081
    Illegal PstI site found at 3374
    Illegal PstI site found at 3540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1636
    Illegal BsaI.rc site found at 598
    Illegal SapI.rc site found at 946


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