Coding

Part:BBa_K4482014

Designed by: Mustafo Mustafokulov   Group: iGEM22_NYC_Empire_State   (2022-10-10)


Human insulin receptor monoclonal antibody (HIRMAb)

The insulin receptor is a transmembrane protein expressed in many different tissues in the human body, including endothelial cells forming the blood vessels in the brain. The insulin receptor takes an active part in transporting insulin from the blood stream into the brain through the Blood-Brain Barrier (BBB). This property of the insulin receptor has been harnessed for the active transport of therapeutic proteins through the BBB. The human insulin receptor monoclonal antibody (HIRMAb) was developed to hitchhike into the insulin receptor and be transported through the BBB to deliver cargo into the brain when injected intravenously. Antibodies are immunoglobulins formed by two different peptides, a heavy chain, and a light chain, that complex together in couples to form the mature antibody capable of recognizing its specific antigen. This part represents the coding sequence for both the heavy and light chain of the HIRMAb recombinant antibody. The sequence was derived from the protein sequence registered in US patent US-8858950-B2.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3501
    Illegal SpeI site found at 821
    Illegal PstI site found at 2910
    Illegal PstI site found at 3076
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 902
    Illegal SpeI site found at 821
    Illegal PstI site found at 2910
    Illegal PstI site found at 3076
    Illegal NotI site found at 3490
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 537
    Illegal BamHI site found at 2813
    Illegal XhoI site found at 575
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3501
    Illegal SpeI site found at 821
    Illegal PstI site found at 2910
    Illegal PstI site found at 3076
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3501
    Illegal SpeI site found at 821
    Illegal PstI site found at 2910
    Illegal PstI site found at 3076
    Illegal AgeI site found at 1006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1516
    Illegal BsaI.rc site found at 598
    Illegal SapI.rc site found at 1864


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