Part:BBa_K4482013

HIR eGFP

The human insulin receptor is a transmembrane receptor that is activated by insulin, IGF-I, IGF-II and belongs to the large class of receptor tyrosine kinase. This receptor plays a large role in glucose homeostasis since insulin signaling controls access to blood glucose in body cells. The sequence coding for the human insulin receptor is attached to the sequence coding for enhanced green fluorescent protein (eGFP) at HIR's C terminus. This part can be transfected into mammalian cells, and through fluorescent microscopy, the presence of HIR can be visualized.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 963
  Illegal PstI site found at 2464
  Illegal PstI site found at 2730
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 963
  Illegal PstI site found at 2464
  Illegal PstI site found at 2730
  Illegal NotI site found at 28
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 963
  Illegal BglII site found at 1532
  Illegal BamHI site found at 1878
  Illegal BamHI site found at 4154
  Illegal XhoI site found at 286
  Illegal XhoI site found at 2043
  Illegal XhoI site found at 3056
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 963
  Illegal PstI site found at 2464
  Illegal PstI site found at 2730
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 963
  Illegal PstI site found at 2464
  Illegal PstI site found at 2730
  Illegal NgoMIV site found at 14
  Illegal NgoMIV site found at 2116
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1744
  Illegal BsaI site found at 2054
  Illegal BsaI.rc site found at 221
  Illegal BsaI.rc site found at 796
  Illegal BsaI.rc site found at 1252
  Illegal BsaI.rc site found at 1574
  Illegal BsaI.rc site found at 3151
  Illegal BsaI.rc site found at 3326
  Illegal SapI site found at 267
  Illegal SapI.rc site found at 3333