Coding
Part:BBa_K4482005
Designed by: Mustafo Mustafokulov Group: iGEM22_NYC_Empire_State (2022-08-25)
NTRK3 eGFP
This part is a fusion protein of NTRK3 (BBa_K4482002) with eGFP at the C-terminus of NTRK3. The part was designed in order to identify cells expressing NTRK3 by fluorescent microscopy.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 111
Illegal PstI site found at 489
Illegal PstI site found at 742 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1840
Illegal PstI site found at 111
Illegal PstI site found at 489
Illegal PstI site found at 742 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 111
Illegal PstI site found at 489
Illegal PstI site found at 742 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 111
Illegal PstI site found at 489
Illegal PstI site found at 742
Illegal NgoMIV site found at 134 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2483
Illegal SapI site found at 435
Illegal SapI site found at 1089
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Categories
Parameters
None |