Coding
Part:BBa_K4482004
Designed by: Mustafo Mustafokulov Group: iGEM22_NYC_Empire_State (2022-08-25)
FGFR2 eGFP
FGFR2 (BBa_K4482000) coding sequence was fused to eGFP coding sequence in order to generate a fusion protein with a fluorescent tag at the C-terminus of the FGFR2.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2276
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2276
Illegal NheI site found at 2470 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2276
Illegal BglII site found at 2084 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2276
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2276
Illegal NgoMIV site found at 786
Illegal NgoMIV site found at 1236 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1386
Illegal BsaI.rc site found at 2238
Illegal BsaI.rc site found at 3113
Illegal SapI.rc site found at 106
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Categories
Parameters
None |