Part:BBa_K4482003

Human IR

The human insulin receptor is a transmembrane protein expressed on epithelial cells of different tissues and binds to insulin present in the blood flow. The insulin receptor present in the lumen of blood vessels in the brain is involved in the transport of insulin from the blood into the brain by active transport through the Blood Brain Barrier. The 2022 NYC_Empire_State iGEM team used the human insulin receptor coding sequence to test the integrity and activity of fusion proteins capable of crossing the Blood Brain Barrier.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 963
  Illegal PstI site found at 2464
  Illegal PstI site found at 2730
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 963
  Illegal PstI site found at 2464
  Illegal PstI site found at 2730
  Illegal NotI site found at 28
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 963
  Illegal BglII site found at 1532
  Illegal BamHI site found at 1878
  Illegal XhoI site found at 286
  Illegal XhoI site found at 2043
  Illegal XhoI site found at 3056
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 963
  Illegal PstI site found at 2464
  Illegal PstI site found at 2730
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 963
  Illegal PstI site found at 2464
  Illegal PstI site found at 2730
  Illegal NgoMIV site found at 14
  Illegal NgoMIV site found at 2116
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1744
  Illegal BsaI site found at 2054
  Illegal BsaI.rc site found at 221
  Illegal BsaI.rc site found at 796
  Illegal BsaI.rc site found at 1252
  Illegal BsaI.rc site found at 1574
  Illegal BsaI.rc site found at 3151
  Illegal BsaI.rc site found at 3326
  Illegal SapI site found at 267
  Illegal SapI.rc site found at 3333