Coding

Part:BBa_K4482002

Designed by: Mustafo Mustafokulov   Group: iGEM22_NYC_Empire_State   (2022-08-25)


Human NTRK3

NTRK3 (Neurotrophic Receptor Tyrosine Kinase 3) that is specific to the protein NT-3 (Neurotrophin-3). Neurotrophin-3 is a protein that in humans is encoded by the NTF3 gene. The protein encoded by this gene, NT-3, is a neurotrophic factor in the NGF (Nerve Growth Factor) family of neurotrophins. It is a protein growth factor which has activity on certain neurons of the peripheral and central nervous system; it helps to support the survival and differentiation of existing neurons, and encourages the growth and differentiation of new neurons and synapses. The 2022 NYC Empire State team fused the sequence NT-3 to eGFP and transfected this recombinant plasmid into CHO-K1 cells. Using fluorescence microscopy, we validated that the cells expressed the receptor on their cell membranes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
    Illegal NgoMIV site found at 134
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 435
    Illegal SapI site found at 1089


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Categories
Parameters
None