![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4482000
Designed by: Mustafo Mustafokulov Group: iGEM22_NYC_Empire_State (2022-08-25)
Human FGFR2
FGFR2 (Fibroblast Growth Factor Receptor 2) is a cell receptor that is coded for by the FGFR2 gene and is specific to the protein FGF-2. FGF-2 is a growth factor protein that enhances proliferation and differentiation of cells. The 2022 NYC Empire State team fused the sequence FGF-2 to eGFP and transfected this recombinant plasmid into CHO-K1 cells. Using fluorescence microscopy, we validated that the cells expressed the receptor on their cell membranes.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2276
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2276
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2276
Illegal BglII site found at 2084 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2276
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2276
Illegal NgoMIV site found at 786
Illegal NgoMIV site found at 1236 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1386
Illegal BsaI.rc site found at 2238
Illegal SapI.rc site found at 106
[edit]
Categories
Parameters
None |