Coding

Part:BBa_K4481010

Designed by: Anne Byford   Group: iGEM22_GastonDay-Shangde   (2022-09-30)


scCCL codon optimized for E. coli

The coding sequence for 4-coumarate:CoA ligase is originally from Streptomyces coelicolor. The coding sequence was codon optimized for use in E. coli using the IDT website.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 163
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1095
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None