Device

Part:BBa_K4472999

Designed by: Stine Behrmann   Group: iGEM22_Uni-Hamburg   (2022-10-10)


Ribozyme half 1

The first half of the ribozyme needs to be used with a ribosome binding site upstream of it and a guide RNA downstream of it. In addition the second half needs to be present in the cell as well as the target RNA to express the reporter. If you want to design your own split ribozyme you can use our software: https://2022.igem.wiki/uni-hamburg/software

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None