Device

Part:BBa_K4472998

Designed by: Stine Behrmann   Group: iGEM22_Uni-Hamburg   (2022-10-10)


Ribozyme half 2

The second half of the ribozyme needs to be used with a guide RNA upstream of it and a reporter gene starting with ATG downstream of it. In addition the first half needs to be present in the cell as well as the target RNA to express the reporter. If you want to design your own split ribozyme you can use our software: https://2022.igem.wiki/uni-hamburg/software

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 327
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 229
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None