Composite

Part:BBa_K4463006

Designed by: Qiaowa Gong   Group: iGEM22_XJTLU-CHINA   (2022-09-18)


CsgA-ShMT3

CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of ShMT3, a protein belongs to Metallothionein family, achieved by linking to CsgA.
IGEM_XJTLU 2022 tested the function of CsgA-ShMT3 by measuring the concentration of adsorbed metal ions, such as Cu(II), Cd(II).

Figure1. E.coli expressed ShMT3 protein showed higher adsorption of Cu(II) in 1.8mM CuSO4 solution.
Figure2. E.coli expressed ShMT3 protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.

The results showed that CsgA-MBP3 had improved the ability of E.coli adsorbing metal ion.
We used ICP-MS to measure the concentration of binding Cu(II) and Cd(II) on the surface of E.coli. For detailed information about our measurement method and result analysis, please visit our Experimentsand Resultson wiki.

 

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
chassisE.coli