Coding

Part:BBa_K4461011

Designed by: CHANG, YU-TANG   Group: iGEM22_NYCU-Taipei   (2022-10-09)


Mut-mCerulean+cutting site

To insert other proteins that can fusion with fluorescent protein, we have to mutate the stop codon for the fluorescent protein. Also, for the convenience of inserting proteins, we added cutting site(EcoRI and HindIII) to do enzyme digestion and ligation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 730
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 730
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 730
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 730
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 730
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None