Coding

Part:BBa_K4461004

Designed by: SHIH-HSUN, YANG   Group: iGEM22_NYCU-Taipei   (2022-10-08)


ansB promoter

The ansB encodes for the L-asparaginase II, which is involved in the asp metabolism. After complete glucose depletion at the late log phase, bacteria catabolize amino acids for energy and carbon. The ansB promoter is down-regulated by the fis, which is mainly expressed in the lag phase and early log phase, also it is co-activated by FNR and CRP, which represent the anaerobic growth and the depletion of glucose, respectively.

This is the ansB mRNA expression-Time graph.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None