Coding
Part:BBa_K4461004
Designed by: SHIH-HSUN, YANG Group: iGEM22_NYCU-Taipei (2022-10-08)
ansB promoter
The ansB encodes for the L-asparaginase II, which is involved in the asp metabolism. After complete glucose depletion at the late log phase, bacteria catabolize amino acids for energy and carbon. The ansB promoter is down-regulated by the fis, which is mainly expressed in the lag phase and early log phase, also it is co-activated by FNR and CRP, which represent the anaerobic growth and the depletion of glucose, respectively.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 117
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |