Part:BBa_K4459002
oxygen-insensitive FNR
To make nitrite detection more specific and more accessible, we have to deal with two more things. We notice that FNR is also a transcriptional factor of the nrfA gene. In fact, FNR is acting as a switch for many bacteria from aerobic to anaerobic metabolism. By direct interaction with oxygen, the Fe-S complex of FNR is quickly converted to [3Fe-4S]+ or a [2Fe-2S]2+, inactivating FNR. Thus one obvious limitation of our project is that we have to ensure an anabolic environment, which may increase the failure or bias of the detection. To solve this we try to introduce a modified oxygen-insensitive FNR mutant protein by substituting either Cys-23 or Cys-122 was substituted for Ser and substitution at position 154.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 568
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 568
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 568
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 568
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 568
- 1000COMPATIBLE WITH RFC[1000]
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