Composite

Part:BBa_K4458017

Designed by: Maya Balakumaran   Group: iGEM22_East_Coast_BioCrew   (2022-10-01)


PCB Biosensor(CYP1A1, CYC1, Akaluc, CYC1 Terminator)

It is a PCB Bionsor that uses the CYP1A1 Promoter, which contains multiple dioxin response elements paired with a generic yeast CYC1 Promoter. These promoters drive the expression of the Akaluc Reporter gene in the presence of dioxins or PCBs.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1426
    Illegal XbaI site found at 3411
    Illegal PstI site found at 944
    Illegal PstI site found at 1021
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2620
    Illegal PstI site found at 944
    Illegal PstI site found at 1021
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 670
    Illegal BglII site found at 721
    Illegal BglII site found at 1111
    Illegal BamHI site found at 697
    Illegal BamHI site found at 4344
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1426
    Illegal XbaI site found at 3411
    Illegal PstI site found at 944
    Illegal PstI site found at 1021
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1426
    Illegal XbaI site found at 3411
    Illegal PstI site found at 944
    Illegal PstI site found at 1021
    Illegal NgoMIV site found at 2634
    Illegal AgeI site found at 4279
    Illegal AgeI site found at 4472
    Illegal AgeI site found at 4807
    Illegal AgeI site found at 5254
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1844
    Illegal BsaI.rc site found at 266


[edit]
Categories
Parameters
None