Coding

Part:BBa_K4458015

Designed by: Maya Balakumaran   Group: iGEM22_East_Coast_BioCrew   (2022-10-01)


Akaluc

YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter). "Akaluciferase (Akaluc) is a new Bioluminescent Imaging System with higher signal strength than standard firefly luciferase (Fluc)."

Dominique Bozec, Anirudh Sattiraju, Alexandros Bouras, Joe G Jesu Raj, Daniel Rivera, Yong Huang, Chrystian Junqueira Alves, Rut Tejero, Nadejda M Tsankova, Hongyan Zou, Constantinos Hadjipanayis, Roland H Friedel, Akaluc bioluminescence offers superior sensitivity to track in vivo glioma expansion, Neuro-Oncology Advances, Volume 2, Issue 1, January-December 2020, vdaa134, https://doi.org/10.1093/noajnl/vdaa134 https://www.addgene.org/182212

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 669
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 797
    Illegal AgeI site found at 1132
    Illegal AgeI site found at 1579
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None