Regulatory

Part:BBa_K4458002

Designed by: Maxwell Swann   Group: iGEM22_East_Coast_BioCrew   (2022-09-10)


PM6-OP3 promoter for Rhodococcus

This PM6 promoter was designed by Round et al. in the paper "An Integrative Toolbox for Synthetic Biology in Rhodococcus" (ACS Synth. Biol. 2021, 10, 2383−2395). They presented this promoter as the strongest constitutive for Rhodococcus species.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 13
    Illegal XbaI site found at 234
    Illegal PstI site found at 1
    Illegal PstI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
    Illegal PstI site found at 131
    Illegal NotI site found at 19
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 13
    Illegal XbaI site found at 234
    Illegal PstI site found at 1
    Illegal PstI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 13
    Illegal XbaI site found at 234
    Illegal PstI site found at 1
    Illegal PstI site found at 131
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None