Coding

Part:BBa_K4455007

Designed by: Gabriella Cerna   Group: iGEM22_ASU   (2022-10-11)


Arsenate Reductase Optimized for Chlamydomonas Reinhardtii

Arsenate reductase is an enzyme which plays an important role in arsenic detoxification in many organisms. An initial step in arsenic detoxification involves the reduction of arsenate (AsV) to arsenite (AsIII). Arsenate reductases catalyze this transformation of AsV to AsIII and have been found to be influential in arsenic detoxification in both prokaryotes and eukaryotes.

This part contains the sequence encoding the eukaryotic arsenate reductase from Saccharomyces cerevisiae. It has been optimized for optimal transgene expression in Chlamydomonas reinhardtii through codon optimization and the insertion of introns.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 185
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 665
    Illegal PstI site found at 185
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 185
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 185
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None