Coding

Part:BBa_K4455006

Designed by: Gabriella Cerna   Group: iGEM22_ASU   (2022-10-11)


Phytochelatin Synthase Optimized for Chlamydomonas Reinhardtii

Phytochelatins are a class of heavy metal binding peptides important for the chelation and sequestration of heavy metals in many types of plants. Phytochelatin synthase is a key enzyme implicated in the phytochelatin biosynthesis pathway; phytochelatin synthase catalyzes the production of phytochelatins from glutathione.

This part contains the sequence encoding phytochelatin synthase. It has been optimized for optimal transgene expression in Chlamydomonas reinhardtii through codon optimization and the insertion of introns.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 540
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1364
    Illegal PstI site found at 540
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1673
    Illegal XhoI site found at 280
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 540
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 540
    Illegal NgoMIV site found at 1885
    Illegal AgeI site found at 478
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None