Part:BBa_K4455003
IFV 2A Peptide Optimized for Chlamydomonas Reinhardtii
IFV is a 2A peptide originating from Infectious Flacherie Virus. 2A peptides are short peptides, typically ranging from 18-22 amino acids in length, which facilitate the expression of more than one protein in a single vector.
In viruses, 2A peptides enable the expression of multiple proteins from a single transcript. During translation, ribosomes which encounter 2A peptides will undergo a "ribosomal skipping event," in which a peptide bond fails to be made. This causes a "cleavage" event to take place between the two polyproteins between the 2A peptide sequence, creating two independent polyproteins.
The sequences for 2A peptides found in viruses, like IFV, can be used in synthetic biology to allow the efficient coexpression of genes in novel biological entities, as they do in their native context. In addition, the small size and abundance of 2A peptide sequences available add to their utility in gene editing.
This part contains the sequence for the IFV 2A peptide optimized for usage in Chlamydomonas reinhardtii.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 31
- 1000COMPATIBLE WITH RFC[1000]
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