Coding
Part:BBa_K4453001
Designed by: Hugo Salas Pedroarena Group: iGEM22_UPNAvarra_Spain (2022-10-02)
Human TDC optimized for E. coli
Human Aromatic-L-amino-acid decarboxylase (TDC) catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine and L-5-hydroxytryptophan to serotonin. This entry contains the sequence of human TDC optimized for TDC synthesis in E. coli (via codon optimization software by IDT Technologies), adapted to be used within the Biobrick Assembly Standards.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1506 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NheI site found at 339
Illegal SpeI site found at 1507
Illegal PstI site found at 1521
Illegal NotI site found at 7
Illegal NotI site found at 1514 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal XhoI site found at 360
Illegal XhoI site found at 870 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1507 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 1507
Illegal PstI site found at 1521 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |