Coding

Part:BBa_K4453001

Designed by: Hugo Salas Pedroarena   Group: iGEM22_UPNAvarra_Spain   (2022-10-02)


Human TDC optimized for E. coli

Human Aromatic-L-amino-acid decarboxylase (TDC) catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine and L-5-hydroxytryptophan to serotonin. This entry contains the sequence of human TDC optimized for TDC synthesis in E. coli (via codon optimization software by IDT Technologies), adapted to be used within the Biobrick Assembly Standards.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 339
    Illegal SpeI site found at 1507
    Illegal PstI site found at 1521
    Illegal NotI site found at 7
    Illegal NotI site found at 1514
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal XhoI site found at 360
    Illegal XhoI site found at 870
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1507
    Illegal PstI site found at 1521
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None