Coding

Part:BBa_K4453000

Designed by: Hugo Salas Pedroarena   Group: iGEM22_UPNAvarra_Spain   (2022-10-02)


Human TPH optimized for E. coli

Human Tryptophan 5-Hydroxilase 1 (TPH) oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis. This entry contains the sequence of human TPH optimized for TPH synthesis in E. coli (via codon optimization software by IDT Technologies), adapted to be used within the Biobrick Assembly Standards.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1665
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1666
    Illegal PstI site found at 1680
    Illegal NotI site found at 7
    Illegal NotI site found at 1673
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 1626
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1666
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1666
    Illegal PstI site found at 1680
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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