Part:BBa_K4452010

pLX-B3Ω1

pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)

Bacterial Resistance: Spectinomycin

GoldenBraid compatibility: enzyme in BsmBI, enzyme out BsaI.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SpeI site found at 233
  Illegal PstI site found at 622
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NheI site found at 616
  Illegal SpeI site found at 233
  Illegal PstI site found at 622
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SpeI site found at 233
  Illegal PstI site found at 622
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SpeI site found at 233
  Illegal PstI site found at 622
  Illegal NgoMIV site found at 954
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 253
  Illegal BsaI.rc site found at 596