Composite

Part:BBa_K4451026

Designed by: Brooks J Rady   Group: iGEM22_Sheffield   (2022-09-30)


SDH Cassette

This composite part is a sorbitol 6-phosphate dehydrogenase expression cassette, which uses the T7 promoter and terminator from pET-21a(+) to allow expression of BBa_K4451005 in an IPTG-inducible manner.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
    Illegal XbaI site found at 302
    Illegal SpeI site found at 275
    Illegal PstI site found at 156
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 275
    Illegal PstI site found at 156
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
    Illegal XbaI site found at 302
    Illegal SpeI site found at 275
    Illegal PstI site found at 156
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal XbaI site found at 302
    Illegal SpeI site found at 275
    Illegal PstI site found at 156
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None