Reporter

Part:BBa_K4451022

Designed by: Brooks J Rady   Group: iGEM22_Sheffield   (2022-09-30)


MutaT7 Test Cassette ATA

BBa_K4451022 is a variant of the composite part BBa_K4451021, differing by only two base pairs. Here, the ACG false-start codon of the streptomycin resistance gene BBa_K4451014 has been replaced by ‘ATA’ via site-directed mutagenesis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1599
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1599
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 850
    Illegal XhoI site found at 1731
    Illegal XhoI site found at 1937
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1599
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1599
    Illegal AgeI site found at 561
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None