Regulatory

Part:BBa_K4451018

Designed by: Brooks J Rady   Group: iGEM22_Sheffield   (2022-09-30)


pJKR PLtetO-1 + RBS

BBa_K4451018 contains the anhydrotetracycline-inducible promoter and ribosome-binding site of pJKR-L-TetR (Rogers et al., 2015; Addgene #62562).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 74
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 74
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 74
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 74
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 74
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None