Coding

Part:BBa_K4451004

Designed by: Brooks J Rady   Group: iGEM22_Sheffield   (2022-09-30)


TadA*-T7RNAP BBa_K4451004 encodes an adenosine deaminase-T7 RNA polymerase chimeric protein. TadA* variants were recently developed through directed evolution by Gaudelli et al. (2020) to exhibit higher editing efficiency (in the context of sgRNA-directed base edititing). Here, the evolved TadA* protein has been tethered to the phage-derived T7 RNA polymerase via a (G3S)7 link, for use in Sheffield 2022’s MutaT7-inspired directed evolution system, ‘rEvolver’.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 499
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2448
    Illegal NheI site found at 2496
    Illegal PstI site found at 499
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 499
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 499
    Illegal NgoMIV site found at 1288
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None