Coding

Part:BBa_K4451003

Designed by: Brooks J Rady   Group: iGEM22_Sheffield   (2022-09-30)


evoAPOBEC1-BE4max-T7RNAP

BBa_K4451003 encodes a cytosine deaminase-T7 RNA polymerase chimeric protein. EvoAPOBEC1-BE4max, a variant of the cytosine deaminase APOBEC1, was developed through phage-assisted continuous evolution by Thuronyi et al. (2019). Here, the protein has been tethered to the phage-derived T7 RNA polymerase via a (G3S)7 link, for use in Sheffield 2022’s MutaT7-inspired directed evolution system, ‘rEvolver’.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 658
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2667
    Illegal NheI site found at 2715
    Illegal PstI site found at 658
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 658
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 658
    Illegal NgoMIV site found at 1507
    Illegal AgeI site found at 13
    Illegal AgeI site found at 706
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None