DNA
HXK1

Part:BBa_K4450005

Designed by: Andreas Sagen   Group: iGEM22_UiOslo_Norway   (2022-06-29)

N-acetylglucosamine kinase 1 from Saccharomyces cerevisiae

N-acetylglucosamine kinase 1 ORF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1244
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1244
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1244
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1244
    Illegal AgeI site found at 637
    Illegal AgeI site found at 1231
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Primers for isolation of the gene to express.
fwd: 5'-GAgctagcTGAGGAGGATGAACGACGCATGGTTCATTTAGGTCCAAAAAAACCAC-'
rev: 5'-CAcacgtgtTTAAGCACCGATGATACCAACGG-3'

Source

Saccharomyces Cerevisiae ATCC 201388

References

Functional Parameters

[edit]
Categories
//cds/enzyme
//function/biosynthesis
Parameters
None