DNA
GFA1

Part:BBa_K4450004

Designed by: Andreas Sagen   Group: iGEM22_UiOslo_Norway   (2022-06-29)


Glutamine--fructose-6-phosphate aminotransferase [isomerizing] from Saccharomyces cerevisiae

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] ORF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 973
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 307
    Illegal BglII site found at 970
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 973
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 973
    Illegal AgeI site found at 112
    Illegal AgeI site found at 852
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1184

Profile

Name: GFA1
Base Pairs: 2154 bp
Origin: Saccharomyces Cerevisiae ATCC 201388
Properties: Transfer an amin group from glutamine to D-glucoseamine 6-phosphate

Usage and Biology

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) is the first enzyme branching off glycolysis into the hexosamine biosynthesis pathway. It is responsible for converting fructose-6-phosphate into D-glucosamine 6-phosphate by transfer of an amine group from glutamine.

Functional Parameters

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Categories
Parameters
None