Primer

Part:BBa_K4449005

Designed by: SRIJANI SAHA ,RASHMI RANJAN BEHERA, SATYAM KUMAR SINGH   Group: iGEM22_IISER_Berhampur   (2022-10-04)

pET15b FimH2 F
pET15b FimH2 F is a primer sequence which is a short single-stranded DNA sequence that is used as a starting point for PCR amplification of a gene. Primers effectively "direct" DNA polymerases where to begin replication of DNA, because DNA polymerase can only add additional nucleotides to an existing strand of DNA, but cannot initiate replication. This part will act as our forward primer and has a length of29 bp . It has the Bam H1 restriction enzyme recognition site and a 5’ overhang of 4 bases ‘TCGA’ for increasing the cleavage efficiency of the Restriction enzyme BamHI. The primer will be needed for the PCR amplification of our gene of interest Fim H2.It has 52% GC content with a Tm of 65 degrees Celsius.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None