Coding

Part:BBa_K4449002

Designed by: SRIJANI SAHA   Group: iGEM22_IISER_Berhampur   (2022-09-28)

Bhp

This DNA part encodes a virulence factor of S. epidermidis that helps in biofilm accumulation and is known as a surface adhesion protein or Bap (biofilm associated protein) like Bhp that is commonly found in S. epidermidis strains. In our project this part will be expressed under the control of the T7 promoter of pET 28b+ expression vector. We will express this protein with a C terminal 6X His tag in BL21DE3 strain of E coli under IPTG induction and purify the protein using Ni NTA affinity column .

Map of Bhp presented in SnapGene

This part will be used for the negative selection round of ligand based SELEX and will serve as a negative control in our ELISA based binding assays. We have chosen this part from Staphylococcus epidermis because Staphylococcus epidermidis comprises a minority of the microflora of the lower part of the Urethra , and can be expected to be present as a contaminant in the urine cultures.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1254
    Illegal EcoRI site found at 1982
    Illegal XbaI site found at 1792
    Illegal SpeI site found at 229
    Illegal SpeI site found at 607
    Illegal SpeI site found at 1750
    Illegal PstI site found at 380
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1254
    Illegal EcoRI site found at 1982
    Illegal SpeI site found at 229
    Illegal SpeI site found at 607
    Illegal SpeI site found at 1750
    Illegal PstI site found at 380
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1254
    Illegal EcoRI site found at 1982
    Illegal BamHI site found at 1611
    Illegal XhoI site found at 6904
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1254
    Illegal EcoRI site found at 1982
    Illegal XbaI site found at 1792
    Illegal SpeI site found at 229
    Illegal SpeI site found at 607
    Illegal SpeI site found at 1750
    Illegal PstI site found at 380
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1254
    Illegal EcoRI site found at 1982
    Illegal XbaI site found at 1792
    Illegal SpeI site found at 229
    Illegal SpeI site found at 607
    Illegal SpeI site found at 1750
    Illegal PstI site found at 380
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None