Part:BBa_K4449002
Bhp
This DNA part encodes a virulence factor of S. epidermidis that helps in biofilm accumulation and is known as a surface adhesion protein or Bap (biofilm associated protein) like Bhp that is commonly found in S. epidermidis strains. In our project this part will be expressed under the control of the T7 promoter of pET 28b+ expression vector. We will express this protein with a C terminal 6X His tag in BL21DE3 strain of E coli under IPTG induction and purify the protein using Ni NTA affinity column .
This part will be used for the negative selection round of ligand based SELEX and will serve as a negative control in our ELISA based binding assays. We have chosen this part from Staphylococcus epidermis because Staphylococcus epidermidis comprises a minority of the microflora of the lower part of the Urethra , and can be expected to be present as a contaminant in the urine cultures.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1254
Illegal EcoRI site found at 1982
Illegal XbaI site found at 1792
Illegal SpeI site found at 229
Illegal SpeI site found at 607
Illegal SpeI site found at 1750
Illegal PstI site found at 380 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1254
Illegal EcoRI site found at 1982
Illegal SpeI site found at 229
Illegal SpeI site found at 607
Illegal SpeI site found at 1750
Illegal PstI site found at 380 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1254
Illegal EcoRI site found at 1982
Illegal BamHI site found at 1611
Illegal XhoI site found at 6904 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1254
Illegal EcoRI site found at 1982
Illegal XbaI site found at 1792
Illegal SpeI site found at 229
Illegal SpeI site found at 607
Illegal SpeI site found at 1750
Illegal PstI site found at 380 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1254
Illegal EcoRI site found at 1982
Illegal XbaI site found at 1792
Illegal SpeI site found at 229
Illegal SpeI site found at 607
Illegal SpeI site found at 1750
Illegal PstI site found at 380 - 1000COMPATIBLE WITH RFC[1000]
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