Part:BBa_K4449000
FimH1
The DNA part contains the coding sequence of the E.coli UTI89 FimH1 signal peptide and lectin domain which forms a subunit of Type 1 fimbriae, located at the tip of the organelle as an integral part of short fimbriae. These structural organelles help the Uropathogens to mediate specific adhesion to alpha -D-mannoside receptors of the host and represent hair-like structures on the surface of E.coli.
Therefore, FimH1 acts as a receptor-recognition element and hence as an adhesin that is responsible for the pathogenesis in urinary tract infection. This part is retrieved from the plasmid pBAD - FimH -9X His that is deposited in the addgene repository by Per Svenningsen Lab. This part will be expressed in the pET28b+ expression vector under the control of the T7 promoter in a suitable host BL21DE3 strain of E .coli. The protein expressed from this part will be needed to test the binding affinity of the candidate aptamers via the ligand-based SELEX technique.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 238
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 238
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 238
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 238
Illegal AgeI site found at 396 - 1000COMPATIBLE WITH RFC[1000]
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