Coding

Part:BBa_K4439012

Designed by: Charlotte Daumal   Group: iGEM22_EPFL   (2022-09-29)

SR-AS-TEV-10xHis

Abstract

The SR-AS-TEV-10xHis protein was implemented in a pET28a vector. This protein could allow to test the polymerisation of [AS] modules (Part:BBa K3944000) present in recombinant green lacewing silk proteins N[AS]8C (Part:BBa K4439002) to create a very specific binding between two proteins.

No experiments were performed because of the time restriction, however one could do several interesting experiments combining this part with another of part containing recombinant green lacewing silk proteins (Part:BBa_K4439009).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 538
    Illegal BglII site found at 607
    Illegal BglII site found at 724
    Illegal BglII site found at 920
    Illegal BamHI site found at 757
    Illegal XhoI site found at 924
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None