Tag

Part:BBa_K4439003

Designed by: Charlotte Daumal   Group: iGEM22_EPFL   (2022-09-29)

Avitag


Abstract

Avitag is an unique 15 amino acid peptide tag using the biotin ligase (BirA) from E. coli. It allows highly targeted enzymatic conjugation for biotinylation. Avitag creates an ideal presentation for molecular binding interactions. For the scope of HESTIA's project, we used Avitag to allow a strong binding between a recombinant silk protein (Part:BBa K4439007) forming a hydrophobic biofilm and a potential fire-retardancy protein (Part:BBa K4439015) constituting a test for a modular protein coating.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

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