Part:BBa_K4438810

MN4_Target_5

MN4_Target_5(BBa_K4438810) is a single-stranded DNA sequence having 86 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. The 5’ end has few bases complementary to MN4_trigger_5_5’trigger_phi29(BBa_K4438809), the middle region has ssDNA sense T7 promoter sequence and the 5’ end has ssDNA sense broccoli sequence.

Usage and Biology

This part along with MN4_trigger_5_5’trigger_phi29(BBa_K4438809) and Aptamer_MN4(BBa_K4438800) can be used to detect different concentrations of quinine [1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.