DNA

Part:BBa_K4438804

Designed by: Shreyas Samir Parkhie   Group: iGEM22_IISER-Tirupati_India   (2022-10-12)

MN4_Target_2

MN4_Target_2(BBa_K4438804) is a single-stranded DNA sequence having 84 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. The 3’ end has few bases complementary to MN4_trigger_2_w/oPol(BBa_K4438803), the middle region has ssDNA antisense T7 promoter sequence and the 5’ end has broccoli cDNA (antisense) sequence.

Usage and Biology

This part along with MN4_trigger_2_w/oPol(BBa_K4438803) and Aptamer_MN4(BBa_K4438800) can be used to detect different concentrations of quinine [1].

T--IISER-Tirupati India--mn4structure2.png



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.

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Parameters
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