DNA

Part:BBa_K4438202

Designed by: Diya Raj N   Group: iGEM22_IISER-Tirupati_India   (2022-10-12)


Spinach2.4

Spinach 2.4 is a mutant version of Spinach 2.1(BBa_K1330000), made to increase the fluorescent intensity given out by the aptamer on binding with DFHBI.


Usage and Biology

The free energy of the thermodynamic ensemble for the sequence below is predicted to be -34.80 kcal/mol. The tetraloop is highlighted in red text and the inversions are underlined. The sequences highlighted in bold are the aptamer sequence and those flanking are the tRNA scaffold sequence, tRNALys3. The mutation and inversions are indicated in the RNAfold structures using blue circles.

Parts-mod-Figure1 (1).jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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