Composite

Part:BBa_K4430005

Designed by: Ruyi Shi   Group: iGEM22_GYHS   (2022-09-20)


GFP-gp13

It is the key part that is responsible for expressing green fluorescent protein (GFP) fusion protein of gp13 (GFP-gp13). Gp13 is a receptor binding protein (RBP) from the genome of E. coli O157 phage EP335. GFP emits green fluorescence under the confocal laser scanning microscope. Under such microscope, green fluorescence on the cell surface of E. coli O157 after incubation with GFP-gp13 proteins indicates specific binding of the proteins to the bacteria. Researches have demonstrated that GFP-gp13 can bind strongly to the cell surfaces of E. coli O157 strains.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1211
    Illegal BglII site found at 2885
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2063
    Illegal AgeI site found at 1334
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641
    Illegal BsaI.rc site found at 2259


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Categories
Parameters
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