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Part:BBa_K4430000

Designed by: Ruyi Shi   Group: iGEM22_GYHS   (2022-09-20)


gp13

It is the key part that is responsible for expressing gp13. Gp13 is a receptor binding protein (RBP) from the genome of E. coli O157 phage EP335. Two distinct tail fibers were identified in the podovirus EP335 genome: gp12 and gp13. Using fluorescence microscopy, researches revealed how gp13 recognizes strains of E. coli serotypes O157:H7.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 474
    Illegal BglII site found at 2148
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1326
    Illegal AgeI site found at 597
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1522


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