Plasmid

Part:BBa_K4427015

Designed by: Chanjuan Jin   Group: iGEM22_JLU_China   (2022-09-30)


pSB1C3-stuffer-BDH

Here, we transferred the blue light photosensitive protein EL222 downstream to the virulence protein BlrA to cue plasmid transformation and achieve lethality against E. coli via a reporter gene.

part-015.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2861
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2862
    Illegal PstI site found at 2876
    Illegal NotI site found at 7
    Illegal NotI site found at 2869
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 1939
    Illegal BamHI site found at 1165
    Illegal XhoI site found at 1743
    Illegal XhoI site found at 2452
    Illegal XhoI site found at 3893
    Illegal XhoI site found at 4785
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2862
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2862
    Illegal PstI site found at 2876
    Illegal NgoMIV site found at 1313
    Illegal AgeI site found at 1000
    Illegal AgeI site found at 2304
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None