Part:BBa_K4427006
mRFP1
Fos proto-oncogene, AP-1 transcription factor subunit
We performed functional validation of the pro plasmid in a dark room. With reference to the literature we performed cycles of blue light irradiation for 2 hours and dark hours under light-proof conditions for the engineered bacteria for a total of 8 hours in two rounds and a control experiment with complete light-proofing. At the end of the two experimental cycles, the fluorescence was checked using UV light irradiation. The results of the experiment showed that the experimental group showed significant fluorescence, while the control group showed no fluorescence.
(The graph on the left shows a comparison of the fluorescent proteins under UV irradiation, with the blue light irradiated group (left) showing significant fluorescence and the control group (right) showing no fluorescence. (The right graph shows the comparison under the naked eye, the blue light irradiated group (left) appears light pink, the control group (right) is colourless)
To determine whether the protein expressed by the engineered bacteria was the mRFP1 fluorescent protein that we expected, we performed fluorescence spectroscopy on the above bacterial broth that was determined to appear fluorescent by UV irradiation. The results of the experimental data showed that the experimentally obtained fluorescent protein had an absorption wavelength of 612 nm and an emission spectrum of 589 nm, which is consistent with the data related to standard mRFP1.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000COMPATIBLE WITH RFC[1000]
| None |