Part:BBa_K4427004
Adh1
Adh1 (alcohol dehydrogenase) converts the above-mentioned PAD catalyzed by KdcA into the final product 2-PE, a geotactic process that uses the genetically catalyzed product PAD of the former enzyme, which is then converted to the final product 2-PE by Adh1.
SDS-PAGE:
ADH1 coenzyme NADH absorption spectrum
We used acetaldehyde and NADH as reaction substrates and a solution of glycine/sodium hydroxide at pH=10 as a buffer to verify the catalytic effect of ADH1 on this reaction. We used a UV spectrophotometer to measure the absorbance of the solution at reaction times of 0, 3, 6, 9, 12, 15 and 18 min. The results showed that NADH had a high absorbance when irradiated with UV light at a wavelength of 340nm and its content decreased with increasing reaction time, proving that our enzyme was active.
Following the determination of the absorbance spectra of ADH1 coenzyme, we determined that the enzyme concentration dependent enzymatic activity of ADH1 was measured using UV light at a wavelength of 340 nm. We added 3, 4, 4.5, 5 and 6 ul of ADH1 to the substrate and performed five sets of controlled experiments.
The results of the experiments showed that the rate of substrate reduction became increasingly rapid with increasing enzyme concentration at 3-6 ul of enzyme solution, i.e. the higher the enzyme activity.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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