Composite

Part:BBa_K4423021

Designed by: Julia Vogl   Group: iGEM22_BOKU-Vienna   (2022-10-12)


pTEF_MaSp_RPL2Att

This part is the complete expression cassette for main strain spider silk, including the "pTEF"-promoter and the "RPL2Att"-terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1065
    Illegal PstI site found at 1260
    Illegal PstI site found at 1272
    Illegal PstI site found at 1419
    Illegal PstI site found at 1614
    Illegal PstI site found at 1626
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1065
    Illegal PstI site found at 1260
    Illegal PstI site found at 1272
    Illegal PstI site found at 1419
    Illegal PstI site found at 1614
    Illegal PstI site found at 1626
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 819
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1065
    Illegal PstI site found at 1260
    Illegal PstI site found at 1272
    Illegal PstI site found at 1419
    Illegal PstI site found at 1614
    Illegal PstI site found at 1626
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1065
    Illegal PstI site found at 1260
    Illegal PstI site found at 1272
    Illegal PstI site found at 1419
    Illegal PstI site found at 1614
    Illegal PstI site found at 1626
    Illegal NgoMIV site found at 983
    Illegal AgeI site found at 2303
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None