Composite
Purify-Rep

Part:BBa_K4422022

Designed by: Grecia García-Elizalde   Group: iGEM22_UAM   (2022-09-30)


Purification and Reporter Device (Promoter AOX1/ Kozak/ 8X His-tag / Short Flexible Linker Gly-Gly-S

This part allows expression, purification and detection of a protein in Pichia pastoris. To use it, you should do two restriction digestions. One with KpnI and one with XhoI and insert the sequence of the desired protein.

Sal.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal XhoI site found at 1215
    Illegal XhoI site found at 1269
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None