Composite
R&FHis-tag

Part:BBa_K4422017

Designed by: Grecia García-Elizalde   Group: iGEM22_UAM   (2022-09-30)


Removable and flexible purification 8X His-tag (8XHis-tag/Linker/Xa Factor)

Flexible polyhistidine tag, which is flexible preventing steric hindrance. In case the tag has an effect in the protein, it can be removed after purification. This is an ideal tag for peptides, since it can be eliminated in case the flexible linker does not work propertly.

Tag 1 .png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None