Part:BBa_K4422008
3XAla Linker
The use of a three-alanine linker has been reported in some research for the construction of fusion proteins between Snowdrop lectin (Galanthus nivalis agglutinin; GNA) and insecticidal peptides. Linkers or spacers are short amino acid sequences created in nature to separate multiple domains in a single protein. Each peptide is attached to the N-terminus of GNA with a three-alanine linker to allow maximum flexibility.
Trung, N. P., Fitches, E., & Gatehouse, J. A. (2006). A fusion protein containing a lepidopteran-specific toxin from the South Indian red scorpion (Mesobuthus tamulus) and snowdrop lectin shows oral toxicity to target insects. BMC Biotechnology, 6, 1–12. https://doi.org/10.1186/1472-6750-6-18
Pyati, P., Fitches, E., & Gatehouse, J. A. (2014). Optimising expression of the recombinant fusion protein biopesticide ω-hexatoxin-Hv1a/GNA in Pichia pastoris: Sequence modifications and a simple method for the generation of multi-copy strains. Journal of Industrial Microbiology and Biotechnology, 41(8), 1237–1247. https://doi.org/10.1007/s10295-014-1466-8
Reddy Chichili, V. P., Kumar, V., & Sivaraman, J. (2013). Linkers in the structural biology of protein-protein interactions. Protein science : a publication of the Protein Society, 22(2), 153–167. https://doi.org/10.1002/pro.2206
Crasto, C. J., & Feng, J. A. (2000, May). LINKER: a program to generate linker sequences for fusion proteins. Protein Engineering, Design and Selection, 13(5), 309–312. https://doi.org/10.1093/protein/13.5.309
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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